The escalating threat of microplastics pollution in the World Ocean is a pressing concern, with 14 million tons of plastic waste anticipated to triple by 2040 unless immediate measures are implemented. The aim of the present study is to present new insights into microplastic pollution in the marine waters of South Australia. Microplastic samples were collected during January-November 2023 in the Gulf of St Vincent (GSV). Five litres of water from both the surface and bottom horizons were sampled in triplicate using Niskin bottles from the boats RV Bungaree (Flinders University) and RV Diomedea (Environmental Protection Agency, EPA). The samples were processed according to the modified protocol from Leterme et al., 2023. The average of 75.63±11.7 particles·L-1 (±SE, standard error) on the surface and 61.2±13.9 particles·L-1 on the bottom layer in the Gulf of St Vincent were recorded. The most common microplastics were fragments and fibers, with other particles occurring in less than 5% of instances. Raman analysis was used to determine the nature of the microparticles. A total of 137 representative particles were probed, of which 42% were successfully matched with known spectra already listed in the available database. Five types of polymers were identified: polyurethan, polyethylene, polypropylene, polystyrene, polyamide and their copolymers.